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Saliva samples
Buccal cell samples via mouth swabs.
Saliva samples via REAL Saliva DNA sample collection kit.
Saliva samples via Oragene self Collection kits.

References: RBMEG06 (50 EXTRACTIONS)
                       RBMEG07 (160 EXTRACTIONS)

Protocol: click here for the protocol

Features:

• Easy collection, transport and processing.
• Painless, non-invasive collection.
• Preserve saliva samples for over 2 years.
• Saliva is preserved and ready to ship.
• Allows for subsequent isolation of DNA of excellent quality and integrity.

Procedure:

Saliva samples are collected by spitting inside the collection funnel which has been assembled with a collection tube.

After collecting 2ml of saliva, the content of saliva preservation solution are added and mixed with the extracted saliva. The saliva collection tube is sent to the laboratory for DNA isolation and analysis using REALPURE SALIVA KIT.
The preserved samples are stable for mre than 1 year at room temperature and more than 2 years at -20ºc or -80ºC.

References: RBMSAL01 (1 UNIT)
                        RBMSAL010 (10 UNITS)

Protocol: click here for the protocol

This kit is designed for the efficient isolation of genomic and mitochondrial DNA from small samples, such as different kinds of cells and tissues, laser-microdissected samples, small amounts of blood  using a special column design.

The special column design  is connected with a reduced dead volume which allows elution in as little as 10 ml.
membrane which selectively binds the DNA.

This kit provides a rapid method for purification and concentration of high-quality DNA from PCR or enzymatic reactions with an extremely small elution volume of only 10 ml using specials MicroSpin columns.

Applications:

• CR products clean-up, efficient desalting of DNA with the removal of DNA polymerases, primers and free dNTPs.
   
• DNA clean-up from Enzymatic Reactions, including: Desphosphorylation, Restriction enzyme digestion, Ligation, Primed synthesis, End labeling and Nick translation.
   
• Isotope and Dye Removal, efficiently removes unicorporated fluorescent ( i.e., AMCA, FITC, BIO, DIG, Cy3, Cy5, FAM, etc) and radiolabeled dNTP derivats from DNA following in vitro labeling reactions.

Features:

• The microspin columns are designed to allow elution in very small volumes (as little as 10 ml) delivering highly concentrated DNA in high yields.
   
• DNA Size Limits: from 70 pb to 23 kb.
   
• DNA Recovery: tipically, up to 5 mg total DNA per column can be eluted into as little as 10 ml.
   
• The protocol is done is 2 minutes.
   
• Fast procedure and easy handling.
   
• Eluted DNA is well suited for use in DNA ligation, sequencing, labelling, PCR, etc.

References:

 Ref.RBMCS04     50 purifications

 Ref.RBMCS05     250 purifications

Click here for the protocol.

This kit is designed for the rapid purification of highly pure genomic DNA from whole blood, serum, plasma, body fluids and dried blood spots using MicroSpin columns with silica membrane  which selectively binds the DNA.

Applications:

• Genomic, bacterial , viral DNA isolation.
   
• DNA from whole blood (human or animal blood, fresh or frozen).
   
• DNA from whole blood treated with citrate, EDTA, heparin.
   
• DNA from serum, plasma, buffy coat, platelets, body fluids, and dried blood spots.

Features:

• For rapid purification of high-quality, ready-to-use DNA from blood.
   
• Sample size: 300 ml whole blood, serum, plasma, body fluids and dried blood spots.
   
• No organic extraction or alcohol precipitation.
   
• Complete removal  of contaminants and inhibitors for reliable downstream applications.
   
• Typical yield: 6- 9 mg genomic DNA.
   
• Elution volume: 50-200 ml.
   
• High quality DNA obtained that can be directly used in PCR, Southern, any enzymatic reaction, cloning, etc.

References:

RBMEGS08: 50 extractions

RBMEGS09: 250 extractions

Click here for protocol.